GACS: GACS Core: DNA microarrays

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PREFERRED TERM

DNA microarrays

TYPE

Topic

DEFINITION

A new way of studying how large numbers of genes interact with each other and how a cell's regulatory networks control vast batteries of genes simultaneously. The method uses a robot to precisely apply tiny droplets containing functional DNA to glass slides. Researchers then attach fluorescent labels to DNA from the cell they are studying. The labeled probes are allowed to bind to complementary DNA strands on the slides. The slides are put into a scanning microscope that can measure the brightness of each fluorescent dot; brightness reveals how much of a specific DNA fragment is present, an indicator of how active it is.
Large sets of oligonucleotide probes arrayed on a solid support, to which a nucleic acid sample can be hybridized to detect variations in gene expression.
Una nueva forma de estudiar cómo interactúan entre sí un gran número de genes y cómo las redes regulatorias de la célula controlan enormes baterías de genes simultáneamente. Esta técnica crea las micromatrices utilizando un robot para aplicar, con alta precisión, gotas minúsculas que contienen ADN de genes sobre un portaobjetos. Luego los investigadores ponen etiquetas fluorescentes al ADN de la célula que están estudiando y dejan que se hibridice con ADN complementario en el portaobjetos. Después ponen las placas en un microscopio computarizado que mide la intensidad de cada punto fluorescente; la intensidad revela la cantidad de fragmentos específicos de ADN presentes, que es un indicador de la actividad de un gen especifico.

BROADER CONCEPT

RELATED CONCEPTS

DNA-hybridisatie

BELONGS TO GROUP

genetic techniques

IN OTHER LANGUAGES

microarray technology	English
cDNA microarrays
cDNA microarray
microarray hybridization
DNA microarray
microarray hybridization assay
gene chip
DNA microarrays
oligonucleotide arrays
cDNA array
oligonucleotide array
gene expression chip
gene expression chips
cDNA arrays
gene chips
tecnología de micromatriz	Spanish

URI

http://id.agrisemantics.org/gacs/C5037

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EXACT MATCH

http://id.cabi.org/cabt/40167	id.cabi.org
http://lod.nal.usda.gov/nalt/42658	lod.nal.usda.gov