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... > methodology > techniques > genetic techniques and protocols > in situ hybridization > fluorescence in situ hybridization

PREFERRED TERM

fluorescence in situ hybridization

TYPE

Topic

DEFINITION

A physical mapping technique that uses fluorescein tags to detect hybridization of DNA probes with metaphase chromosomes and with the less-condensed somatic interphase chromatin. Used to locate, mark or map DNA sequences, genes, regions or complete chromosomes.
A type of in situ hybridization in which target sequences are stained with fluorescent dye so their location and size can be determined using fluorescence microscopy. This staining is sufficiently distinct that the hybridization signal can be seen both in metaphase spreads and in interphase nuclei.
Tipo de hibridación in situ en la cual secuencias específicas se tiñen con tintura fluorescente para poder determinar su ubicación y tamaño, utilizando microscopía fluorescente. Esta tinción es lo suficientemente clara para poder ver la señal de hibridación en los despliegues de la metafase y en los núcleos de la interfase.

BROADER CONCEPT

in situ hybridization

RELATED CONCEPTS

ALTERNATIVE LABEL

FISH (cytogenetic techniques)
FISH (fluorescence in situ hybridisation)
FISH (fluorescence in situ hybridization)
FISH (fluorescent in situ hybridisation)
FISH (fluorescent in situ hybridization)
fluorescence in situ hybridisation
fluorescent in-situ hybridization
fluorescent in situ hybridisation
fluorescent in situ hybridization

EDITORIAL NOTE

Acronym is FISH.

BELONGS TO GROUP

genetic techniques

IN OTHER LANGUAGES

fluorescentie in situ hybridisatie	Dutch
hibridación in situ fluorescente	Spanish

URI

http://id.agrisemantics.org/gacs/C8244

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EXACT MATCH

http://id.cabi.org/cabt/48709	id.cabi.org
http://lod.nal.usda.gov/nalt/40326	lod.nal.usda.gov